She was arrested and brought in for questioning after the crash Friday, Ottawa police said Saturday.
The crash took place west of downtown at 3:50 p.m., at the beginning of rush hour, just as dusk was beginning to settle on the coldest day of the winter so far in the capital.
The Ottawa Hospital issued an update on the status of patients they received.
People are being asked to avoid the area as crews help those who have been injured.
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Const. Amy Gagnon says officers worked through the night and identified the three victims and contacted their families.
In a press conference, Ottawa's Police Chief said the most serious injuries involved people sitting on the second-level of the bus.
She says their names will not be released at this time.
Ontario Premier Doug Ford said: "Devastated to hear about those who lost their lives and were injured following the horrific bus collision at Westboro Station in Ottawa".
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Brad Muir, a retired sergeant with the Ontario Provincial Police and a collision reconstruction consultant, told CTV News Channel that investigators will likely be downloading electronic data from the bus and reviewing eyewitness statements, road conditions and video camera surveillance. "I have asked staff to ensure all necessary resources are made available to assist them through this hard time". "I imagine there's still some people to be interviewed, given the large number of people on the bus".
OC Transpo, which is in charge of Ottawa's public transportation, tweeted on its verified account, "Buses are being detoured as a result" of the collision.
Witnesses said first responders had to break windows and use ladders to free passengers trapped on the upper deck of the bus.
In 2013, another Ottawa double-decker city bus broke through a warning gate and collided with a Via Rail train in suburban Barrhaven, killing six people as the train sheared off the front of the vehicle.
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